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Test Code C AFB Culture, Mycobacterium (AFB)

Methodology

Culture with Acid Fast Stain Included

Specimen Requirements

Note: As with other cultures, the administration of antimicrobial therapy prior to culture may make the recovery of pathogens difficult to impossible.

 

1.  Specimen Source is Required.

2.  Refer to the Microbiology Specimen Collection Procedure for collection instructions.

 

 

Acceptable Specimens: Body Fluid, Tissue, Bones, Blood, Bronchial Alveolar Lavage, Bronchial Brush/Wash, Sputum, Stool, Urine, Wound

 

Unacceptable Specimens: Swab Collections

Day(s) Test Set Up

Monday through Sunday

Reference Values

ACID-FAST BACILLI CULTURE

No acid-fast bacilli isolated after 8 weeks

Critical value (automatic call-back): positive AFB growth

 

ACID-FAST BACILLI SMEAR

No acid-fast bacilli seen

Critical value (automatic call-back): all positive smears

Test Classification and CPT Coding

87015-Concentration for infectious agent

87116-Culture

87206-Acid-fast bacilli smear

Performing Laboratory

Beebe Healthcare Laboratory

Performing Location

Margaret H. Rollins Laboratory

Specimen Transport Temperature

Refrigerate

Additional Information

Cultures are worked up and reported per standard Microbiology guidelines.  Full organism identification and antimicrobial susceptibility testing are performed when appropriate and will incur additional CPT codes and charges.  Pertinent patient history should be communicated directly to the Microbiology Department when necessary (patient travel history, suspect and/or rule out certain pathogens, any unusual circumstances, etc) so that the culture can be worked up appropriately.  If additional workup is required beyond the final culture report, please contact the Microbiology Department at (302)-645-3563 within 5 days to discuss the case with a technologist.