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HelpTest Code BRUTA Brucella Total Antibody Confirmation, Agglutination, Serum
Reporting Name
Brucella Ab, Agglutination, SUseful For
Diagnosis of brucellosis
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumSpecimen Required
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.25 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 14 days |
Reference Values
<1:80
Day(s) Performed
Wednesday, Friday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86622
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| BRUTA | Brucella Ab, Agglutination, S | 19053-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 8112 | Brucella Ab, Agglutination, S | In Process |
Interpretation
The Centers for Disease Control and Prevention recommends that specimens testing positive or equivocal for IgG or IgM by a screening enzyme immunoassay (EIA) be confirmed by a Brucella-specific agglutination method.
Titers below 1:80 are seen in normal, healthy populations. Titers of 1:80 or greater are often considered clinically significant (1); however, a 4-fold or greater increase in titers between acute and convalescent phase sera is required to diagnose acute infection.
Positive results by a screening EIA that are not confirmed by Brucella-specific agglutination may represent false-positive screening results. If clinically indicated, a new specimen should be tested after 7 to 14 days.
Clinical Reference
1. Welch RJ, Litwin CM. A comparison of Brucella IgG and IgM ELISA assays with agglutination methodology. J Clin Lab Anal. 2010;24(3):160-162
2. Gunes H, Dogan M False-positivity in diagnosis of brucellosis associated with Rev-1 vaccine. Libyan J Med. 2013:8:10.3402/ljm.v8i0.20417
3. Stoddard RA. Detection of Brucella spp antibodies. In: Leber AL, Burnham CD, eds. Clinical Microbiology Procedures Handbook. 5th ed. AMS Press; 2023:section13.3.3
Report Available
2 to 7 daysMethod Name
Agglutination
Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.