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Test Code BMIYC Borrelia miyamotoi Detection PCR, Spinal Fluid

Useful For

Aids in the diagnosis of Borrelia miyamotoi infection in conjunction with clinical findings

 

This test is not useful for detecting the Borrelia species that cause Lyme disease.

Method Name

Real-Time Polymerase Chain Reaction (PCR)

Reporting Name

Borrelia miyamotoi Detection PCR, C

Specimen Type

CSF


Ordering Guidance


This assay does not detect the Borrelia species that cause Lyme disease.



Specimen Required


Container/Tube: Sterile vial

Specimen Volume: 1 mL

Collection Instructions: Submit aliquot from collection vial 2.


Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time
CSF Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Negative

Interpretation

A positive result indicates the presence of Borrelia miyamotoi DNA and is consistent with active or recent infection. While positive results are highly specific indicators of disease, they should be correlated with symptoms and clinical findings of tick-borne relapsing fever. Less commonly, this test may also detect the nucleic acid of other relapsing fever borreliae; when detected, the report indicates the presence of relapsing fever Borrelia species, not B miyamotoi.

Clinical Reference

1. Centers for Disease Control and Prevention. About Hard Tick Relapsing Fever. CDC; 2024. Accessed December 30, 2025. Available at www.cdc.gov/relapsing-fever/about/about-htrf.html

2. Hoornstra D, Azagi T, van Eck JA, et al. Prevalence and clinical manifestation of Borrelia miyamotoi in Ixodes ticks and humans in the northern hemisphere: a systematic review and meta-analysis. Lancet Microbe. 2022;3(10):e772-e786. doi:10.1016/S2666-5247(22)00157-4

3. Miller JM, Binnicker MJ, Campbell S, et al. Guide to Utilization of the Microbiology Laboratory for Diagnosis of Infectious Diseases: 2024 Update by the Infectious Diseases Society of America (IDSA) and the American Society for Microbiology (ASM). Clin Infect Dis. Published online March 5, 2024. doi:10.1093/cid/ciae104

4. Kubiak JM, Klevay M, Hilt EE, Ferrieri P. Acute meningoencephalitis associated with Borrelia miyamotoi, Minnesota, USA. Emerg Infect Dis. 2024;30(7):1472-1474. doi:10.3201/eid3007.231611

5. Rodino KG, Pritt BS. When to think about other borreliae:: Hard tick relapsing fever (Borrelia miyamotoi), Borrelia mayonii, and beyond. Infect Dis Clin North Am. 2022;36(3):689-701. doi:10.1016/j.idc.2022.04.002

Day(s) Performed

Monday through Sunday

Report Available

1 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87478

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BMIYC Borrelia miyamotoi Detection PCR, C 82476-3

 

Result ID Test Result Name Result LOINC Value
64969 B. miyamotoi PCR, C 82476-3

Forms

If not ordering electronically, complete, print, and send Microbiology Test Request (T244) with the specimen.

Testing Algorithm

For more information see Acute Tick-Borne Disease Testing Algorithm