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Test Code BMIPB Borrelia miyamotoi Detection, PCR, Blood


Ordering Guidance


This assay does not detect the Borrelia species that cause Lyme disease. If Lyme disease is suspected, order SLYME / Lyme Antibody Modified 2-Tier with Reflex, Serum.



Specimen Required


Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Royal blue top (EDTA), pink top (EDTA), or sterile vial containing EDTA-derived aliquot

Specimen Volume: 1 mL

Collection Instructions: Send whole blood specimen in original tube (preferred).


Useful For

Aiding in the diagnosis of Borrelia miyamotoi infection in conjunction with clinical findings

 

Preferred method for detection of B miyamotoi using blood specimens

Testing Algorithm

For information see Acute Tickborne Disease Testing Algorithm.

Method Name

Real-Time Polymerase Chain Reaction (PCR)

Reporting Name

Borrelia miyamotoi Detection, PCR, B

Specimen Type

Whole Blood EDTA

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood EDTA Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Negative

Reference values apply to all ages.

Interpretation

A positive result indicates the presence of Borrelia miyamotoi DNA and is consistent with active or recent infection. While positive results are highly specific indicators of disease, they should be correlated with symptoms and clinical findings of tick-borne relapsing fever.

Clinical Reference

1. Hoornstra D, Azagi T, van Eck JA, et al. Prevalence and clinical manifestation of Borrelia miyamotoi in Ixodes ticks and humans in the northern hemisphere: a systematic review and meta-analysis. Lancet Microbe. 2022;3(10):e772-e786

2. McCormick DW, Brown CM, Bjork J, et al. Characteristics of hard tick relapsing fever caused by borrelia miyamotoi, United States, 2013-2019. Emerg Infect Dis. 2023;29(9):1719-1729

3. Xu G, Luo CY, Ribbe F, et al. Borrelia miyamotoi in human-biting ticks, United States, 2013-2019. Emerg Infect Dis. 2021;27(12):3193-3195

4. Kingry LC, Anacker M, Pritt B, et al. Surveillance for and discovery of borrelia species in US patients suspected of tickborne illness. Clin Infect Dis. 2018;66(12):1864-1871

5. Wormser GP, Shapiro ED, Fish D. Borrelia miyamotoi: an emerging tick-borne pathogen. Am J Med. 2019;132(2):136-137

6. Telford SR, Goethert HK, Molloy PJ, Berardi V. Blood smears have poor sensitivity for confirming borrelia miyamotoi disease. J Clin Microbiol. 2019;57(3):e01468-18. Published 2019 Feb 27. doi:10.1128/JCM.01468-18

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87478

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BMIPB Borrelia miyamotoi Detection, PCR, B 82475-5

 

Result ID Test Result Name Result LOINC Value
618298 B. miyamotoi PCR, B 82475-5

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.