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HelpTest Code BFLA1 Lipid Analysis, Body Fluid
Necessary Information
1. Date and time of collection are required.
2. Specimen source is required.
Specimen Required
Specimen Type: Body fluid
Preferred Sources:
-Peritoneal fluid (peritoneal, abdominal, ascites, paracentesis)
-Pleural fluid (pleural, chest, thoracentesis)
-Drain fluid (drainage, JP drain)
-Pericardial
Acceptable Source:
Collection Container/Tube: Sterile container, no additive
Submission Container/Tube: Plastic vial
Specimen Volume: 3 mL
Collection Instructions:
1. Centrifuge to remove any cellular material and transfer into a plastic vial.
2. Indicate the specimen source and source location on label.
Useful For
Distinguishing between chylous and nonchylous effusions
Method Name
Colorimetry/Spectrophotometry (SP)/Electrophoresis
Reporting Name
Lipid Analysis, BFSpecimen Type
Body FluidSpecimen Minimum Volume
2.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Body Fluid | Frozen (preferred) | 30 days |
| Refrigerated | 7 days | |
| Ambient | 24 hours |
Reference Values
An interpretive report will be provided.
Interpretation
Pleural Fluid:
Pleural fluid cholesterol concentrations 46 to 65 mg/dL are consistent with exudative effusions.
Cholesterol concentrations greater than 200 mg/dL suggest pseudochylous effusion.
Triglyceride concentrations greater than 110 mg/dL are consistent with chylous effusions.
Triglyceride concentrations less than 50 mg/dL are usually not due to chylous effusions.
Peritoneal Fluid:
Peritoneal fluid triglyceride concentrations greater than 187 mg/dL are most consistent with chylous effusion.
Cholesterol concentrations 33 to 70 mg/dL suggest malignant causes of ascites.
Clinical Reference
1. Hooper C, Lee YC, Maskell N; BTS Pleural Guideline Group. Investigation of a unilateral pleural effusion in adults: British Thoracic Society Pleural Disease Guideline 2010. Thorax. 2010;65 Suppl 2:ii4-ii17
2. Staats BA, Ellefson RD, Budahn LL, Dines DE, Prakash UB, Offord K. The lipoprotein profile of chylous and nonchylous pleural effusions. Mayo Clin Proc. 1980;55(11):700-704
3. Thaler MA, Bietenbeck A, Schulz C, Luppa PB. Establishment of triglyceride cut-off values to detect chylous ascites and pleural effusions. Clin Biochem. 2017;50(3) 134-138
4. Burtis CA, Ashwood ER, Bruns DE, Tietz NW, eds. In: Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 5th ed. Elsevier; 2012:1 (xviii, 2238)
5. Noble RP. Electrophoretic separation of plasma lipoproteins in agarose gel. J Lipid Res 1968;9(6):693-700
6. Sepiashvili L, Dahl AR, Meeusen JM, Loftus CG, Donato LJ. A man with recurrent ascites after laparoscopic cholecystectomy. Clin Chem. 2017;63(7):1199-1203
7. Ellefson RD, Elveback L, Weidman W. Application of methods used for lipoprotein analysis: plasma lipoproteins of children and youths in Rochester, MN. DHEW Publication No. (NIH). 1978;78-1472
Day(s) Performed
Monday through Friday
Report Available
3 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
82664-Electrophoretic technique, not elsewhere specified (Chylomicrons and lipoproteins)
84311-Spectrophotometry, analyte not specified (Cholesterol)
84478-Triglycerides
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| BFLA1 | Lipid Analysis, BF | 94872-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| FLD28 | Fluid Type: | 14725-6 |
| BFCHO | Cholesterol, BF | 12183-0 |
| BFTRG | Triglycerides, BF | 12228-3 |
| BFCMT | Comment | 21025-2 |