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Test Code BFLA1 Lipid Analysis, Body Fluid


Necessary Information


1. Date and time of collection are required.

2. Specimen source is required.



Specimen Required


Specimen Type: Body fluid

Preferred Sources:

-Peritoneal fluid (peritoneal, abdominal, ascites, paracentesis)

-Pleural fluid (pleural, chest, thoracentesis)

-Drain fluid (drainage, JP drain)

-Pericardial

Acceptable Source:

Collection Container/Tube: Sterile container, no additive

Submission Container/Tube: Plastic vial

Specimen Volume: 3 mL

Collection Instructions:

1. Centrifuge to remove any cellular material and transfer into a plastic vial.

2. Indicate the specimen source and source location on label.


Useful For

Distinguishing between chylous and nonchylous effusions

Method Name

Colorimetry/Spectrophotometry (SP)/Electrophoresis

Reporting Name

Lipid Analysis, BF

Specimen Type

Body Fluid

Specimen Minimum Volume

2.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Body Fluid Frozen (preferred) 30 days
  Refrigerated  7 days
  Ambient  24 hours

Reference Values

An interpretive report will be provided.

Interpretation

Pleural Fluid:

Pleural fluid cholesterol concentrations 46 to 65 mg/dL are consistent with exudative effusions.

Cholesterol concentrations greater than 200 mg/dL suggest pseudochylous effusion.

Triglyceride concentrations greater than 110 mg/dL are consistent with chylous effusions.

Triglyceride concentrations less than 50 mg/dL are usually not due to chylous effusions.

 

Peritoneal Fluid:

Peritoneal fluid triglyceride concentrations greater than 187 mg/dL are most consistent with chylous effusion.

Cholesterol concentrations 33 to 70 mg/dL suggest malignant causes of ascites.

Clinical Reference

1. Hooper C, Lee YC, Maskell N; BTS Pleural Guideline Group. Investigation of a unilateral pleural effusion in adults: British Thoracic Society Pleural Disease Guideline 2010. Thorax. 2010;65 Suppl 2:ii4-ii17

2. Staats BA, Ellefson RD, Budahn LL, Dines DE, Prakash UB, Offord K. The lipoprotein profile of chylous and nonchylous pleural effusions. Mayo Clin Proc. 1980;55(11):700-704

3. Thaler MA, Bietenbeck A, Schulz C, Luppa PB. Establishment of triglyceride cut-off values to detect chylous ascites and pleural effusions. Clin Biochem. 2017;50(3) 134-138

4. Burtis CA, Ashwood ER, Bruns DE, Tietz NW, eds. In: Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 5th ed. Elsevier; 2012:1 (xviii, 2238)

5. Noble RP. Electrophoretic separation of plasma lipoproteins in agarose gel. J Lipid Res 1968;9(6):693-700

6. Sepiashvili L, Dahl AR, Meeusen JM, Loftus CG, Donato LJ. A man with recurrent ascites after laparoscopic cholecystectomy. Clin Chem. 2017;63(7):1199-1203

7. Ellefson RD, Elveback L, Weidman W. Application of methods used for lipoprotein analysis: plasma lipoproteins of children and youths in Rochester, MN. DHEW Publication No. (NIH). 1978;78-1472

Day(s) Performed

Monday through Friday

Report Available

3 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

82664-Electrophoretic technique, not elsewhere specified (Chylomicrons and lipoproteins)

84311-Spectrophotometry, analyte not specified (Cholesterol)

84478-Triglycerides

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BFLA1 Lipid Analysis, BF 94872-9

 

Result ID Test Result Name Result LOINC Value
FLD28 Fluid Type: 14725-6
BFCHO Cholesterol, BF 12183-0
BFTRG Triglycerides, BF 12228-3
BFCMT Comment 21025-2