Sign in →

Test Code BETV2 BET v2 (Profilin), IgE, Serum


Ordering Guidance


For a listing of allergens available for testing, see Allergens - Immunoglobulin E (IgE) Antibodies.

 

BET v2 is also available as part of the PEANT / Peanut, IgE with Reflex to Peanut Components, IgE, Serum. This reflex test includes Ara h2, h1, h3, h6, h9, and Bet v2 (profilin) if the initial Peanut IgE test is detectable.



Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL for every 5 allergens requested

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Forms

If not ordering electronically, complete, print, and send an Allergen Test Request (T236) with the specimen.

Useful For

Evaluation of patients suspected birch pollen allergy

 

Evaluation of patients with suspected peanut allergy

 

Evaluation of patients with oral allergy syndrome to other pollens or plant-based foods

Method Name

Fluorescence Enzyme Immunoassay (FEIA)

Reporting Name

BET v2 (Profilin), IgE, S

Specimen Type

Serum

Specimen Minimum Volume

For 1 allergen: 0.3 mL
For more than 1 allergen: (0.05 mL x number of allergens) + 0.25 mL deadspace

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  90 days
  Ambient  7 days

Reference Values

Class

IgE kU/L

Interpretation

0

<0.10

Negative

0/1

0.10-0.34

Borderline/Equivocal

1

0.35-0.69

Equivocal

2

0.70-3.49

Positive

3

3.50-17.4

Positive

4

17.5-49.9

Strongly positive

5

50.0-99.9

Strongly positive

6

≥100

Strongly positive

Concentrations ≥0.70 kU/L (Class 2 and above) will flag as abnormally high.

Interpretation

Profilins are potentially cross-reactive allergenic proteins found in many plant pollens and tissues. IgE antibodies to the profilin Bet v2, while associated with birch pollen sensitivity, also represent a minor peanut allergen marker as it is cross-reactive with the peanut profilin Ara h5. The presence of antibodies to profilin Bet v2 is typically associated with milder allergic reactions and oral allergy syndrome.

Clinical Reference

1. Anhoej C, Backer V, Nolte H: Diagnostic evaluation of grass- and birch-allergic patients with oral allergy syndrome. Allergy. 2001 Jun;56(6):548-552. doi: 10.1034/j.1398-9995.2001.056006548.x

2. Sekerkova A, Polackova M: Detection of Bet v1, Bet v2 and Bet v4 specific IgE antibodies in the sera of children and adult patients allergic to birch pollen: evaluation of different IgE reactivity profiles depending on age and local sensitization. Int Arch Allergy Immunol. 2011;154(4):278-85. doi: 10.1159/000321819

3. D'Amato G, Cecchi L, Bonini S, et al: Allergenic pollen and pollen allergy in Europe. Allergy. 2007 Sep;62(9):976-990. doi: 10.1111/j.1398-9995.2007.01393.x

4. Cabanos C, Tandang-Silvas MR, Odijk V, et al: Expression, purification, cross-reactivity and homology modeling of peanut profilin. Protein Expr Purif. 2010 Sep;73(1):36-45. doi: 10.1016/j.pep.2010.03.005

5. Bublin M, Breiteneder H: Cross-reactivity of peanut allergens. Curr Allergy Asthma Rep. 2014 Apr;14(4):426. doi: 10.1007/s11882-014-0426-8

6. Chan ES, Greenhawt MJ, Fleischer DM, Caubet JC: Managing cross-reactivity in those with peanut allergy. J Allergy Clin Immunol Pract. 2019 Feb;7(2):381-386. doi: 10.1016/j.jaip.2018.11.012

7. Simberloff T, Parambi R, Bartnikas LM, et al: Implementation of a standardized clinical assessment and management plan (SCAMP) for food challenges. J Allergy Clin Immunol Pract. 2017 Mar-Apr;5(2):335-344.e3. doi:10.1016/j.jaip.2016.05.021

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86008

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BETV2 BET v2 (Profilin), IgE, S 30985-6

 

Result ID Test Result Name Result LOINC Value
BETV2 BET v2 (Profilin), IgE, S 30985-6

Day(s) Performed

Monday through Friday

Report Available

Same day/1 to 3 days