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HelpTest Code BAPS Bile Acid Profile, Serum
Ordering Guidance
This test is intended for use by research scientists. Approval must be obtained before ordering.
Specimen Required
Patient Preparation: Patient must be fasting for 12 to 14 hours.
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Useful For
Evaluating the enterohepatic cycle consisting of the biliary system, intestine, portal circulation, and hepatocytes
Supporting researchers in need of free and conjugated values of all 20 bile acid species as well as total bile acid
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Bile Acid Profile, SSpecimen Type
SerumSpecimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 90 days | |
| Ambient | 90 days | ||
| Frozen | 90 days |
Reference Values
Chenodeoxycholic acid: ≤2.26 nmol/mL
Cholic acid: ≤2.74 nmol/mL
Deoxycholic acid: ≤2.84 nmol/mL
Dihydroxycholestanoic acid: ≤0.07 nmol/mL
Glycochenodeoxycholic acid: ≤5.14 nmol/mL
Glycocholic acid: ≤2.17 nmol/mL
Glycodeoxycholic acid: ≤3.88 nmol/mL
Glycohyodeoxycholic acid: ≤0.01 nmol/mL
Glycolithocholic acid: ≤0.11 nmol/mL
Glycoursodeoxycholic acid: ≤1.00 nmol/mL
Hyodeoxycholic acid: ≤0.12 nmol/mL
Lithocholic acid: ≤0.09 nmol/mL
Taurochenodeoxycholic acid: ≤0.80 nmol/mL
Taurocholic acid: ≤0.31 nmol/mL
Taurodeoxycholic acid: ≤0.78 nmol/mL
Taurohyodeoxycholic acid: ≤0.02 nmol/mL
Taurolithocholic acid: ≤0.04 nmol/mL
Tauroursodeoxycholic acid: ≤0.05 nmol/mL
Trihydroxycholestanoic acid: ≤1.73 nmol/mL
Ursodeoxycholic acid: ≤0.64 nmol/mL
Total bile acids: ≤19.00 nmol/mL
Interpretation
Total bile acids are metabolized in the liver and can serve as a marker for normal liver function. Increases in serum C27 bile acids are seen in patients with peroxisomal biogenesis disorders (eg, as Zellweger spectrum disorder) or single enzyme defects of bile acid synthesis (eg, D-bifunctional protein deficiency and alpha methyl CoA racemases).
Totals of the free and conjugated bile acid species for all 20 bile acids in addition to total bile acids will be reported. No interpretive report will be provided.
Clinical Reference
1. Sundaram SS, Bove KE, Lovell MA, Sokol RJ. Mechanisms of disease: inborn errors of bile acid synthesis. Nat Clin Pract Gastroenterol Hepatol. 2008;5(8):456-468
2. Wanders RJA, Rizzo WB. Inborn errors of peroxisome biogenesis and function. In: Sarafoglou K, Hoffmann GF, Roth KS, eds. Pediatric Endocrinology and Inborn Errors of Metabolism. McGraw-Hill Medical Division. 2nd ed. 2017:427-446
3. Ducroq DH, Morton MS, Shadi N, et al. Analysis of serum bile acids by isotope dilution-mass spectrometry to assess the performance of routine total bile acid methods. Ann Clin Biochem. 2010;47(Pt 6):535-540
4. Fischler B, Eggersten G, Bjorkhem I. Genetic defects in synthesis and transport of bile acids. In: Sarafoglou K, Hoffmann GF, Roth KS, eds. Pediatric Endocrinology and Inborn Errors of Metabolism. McGraw-Hill Medical Division; 2017:447-460
5. Society for Maternal-Fetal Medicine (SMFM). Lee RH, Mara Greenberg, Metz TD, Pettker CM. Society for Maternal-Fetal Medicine Consult Series #53: Intrahepatic cholestasis of pregnancy: replaces consult #13, April 2011. Am J Obstet Gynecol. 2021;224(2):B2-B9. doi:10.1016/j.ajog.2020.11.002
Day(s) Performed
Monday through FridayReport Available
3 to 5 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
82542
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| BAPS | Bile Acid Profile, S | 43130-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 35802 | Chenodeoxycholic acid | 30519-3 |
| 35801 | Cholic acid | 30518-5 |
| 35803 | Deoxycholic acid | 30520-1 |
| 35819 | Dihydroxycholestanoic acid | 53479-2 |
| 35808 | Glycochenodeoxycholic acid | 93335-8 |
| 35807 | Glycocholic acid | 93334-1 |
| 35809 | Glycodeoxycholic acid | 93333-3 |
| 35811 | Glycohyodeoxycholic acid | 93332-5 |
| 35812 | Glycolithocholic acid | 93331-7 |
| 35810 | Glycoursodeoxycholic acid | 93330-9 |
| 35805 | Hyodeoxycholic acid | 93329-1 |
| 35806 | Lithocholic acid | 74897-0 |
| 35814 | Taurochenodeoxycholic acid | 93328-3 |
| 35813 | Taurocholic acid | 93327-5 |
| 35815 | Taurodeoxycholic acid | 93326-7 |
| 35817 | Taurohyodeoxycholic acid | 93325-9 |
| 35818 | Taurolithocholic acid | 93324-2 |
| 35816 | Tauroursodeoxycholic acid | 93323-4 |
| 35820 | Trihydroxycholestanoic acid | 38188-9 |
| 35804 | Ursodeoxycholic acid | 55159-8 |
| 35821 | Total bile acids | 14628-2 |