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MEDIALAB

COURIER SERVICE INFORMATION

SPECIMEN DROP-OFF INFORMATION FOR PROVIDERS

MCE Electronic Courier Tracking Information 

HOW TO REQUEST LAB SUPPLIES

HELPFUL LINKS

Mayo Clinic Laboratories

Test Code APCRR Activated Protein C Resistance V, with Reflex to Factor V Leiden, Blood and Plasma

Useful For

Evaluating patients with incident or recurrent venous thromboembolism (VTE)

 

Evaluating individuals with a family history of VTE

Profile Information

Test ID Reporting Name Available Separately Always Performed
APCRV Activated Protein Resistance V, P Yes Yes
SC018 Whole Blood No Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
F5DNA Factor V Leiden (R506Q) Mutation, B Yes No
F5DNI APCRV/F5DNA Summary Interpretation No No

Testing Algorithm

If the assay ratio is abnormal, then factor V Leiden variant analysis will be performed at an additional charge.

 

When the activated protein C resistance V is abnormal or indeterminate and the factor V Leiden variant assay is performed, a summary interpretation will be provided.

Method Name

Optical Clot-Based

Reporting Name

APCRV, w/Reflex, P

Specimen Type

Plasma Na Cit
Whole blood


Necessary Information


 



Specimen Required


Blood and plasma are required.

 

Patient Preparation: Fasting preferred

 

Specimen Type: Whole blood

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD) or light-blue top (3.2% sodium citrate)

Specimen Volume: 3 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send whole blood specimen in original tube. Do not aliquot.

 

Specimen Type: Platelet-poor plasma

Collection Container/Tube: Light-blue top (3.2% sodium citrate)

Submission Container/Tube: Plastic vial, polypropylene preferred

Specimen Volume: 1 mL

Collection Instructions:

1. For complete instructions, see Coagulation Guidelines for Specimen Handling and Processing.

2. Within 4 hours of collection, centrifuge, transfer all plasma into a plastic vial, and centrifuge plasma again.

3. Aliquot plasma into separate plastic vial leaving 0.25 mL in the bottom of centrifuged vial.

4. Freeze plasma aliquot immediately at -20° C, or ideally, at -40° C or below.

Additional Information:

1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

2. Each coagulation assay requested should have its own vial.


Specimen Minimum Volume

Plasma: 0.5 mL; Whole blood: 3 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma Na Cit Frozen 14 days
Whole blood Ambient (preferred) 14 days
  Frozen  14 days
  Refrigerated  14 days

Reference Values

ACTIVATED PROTEIN C RESISTANCE V RATIO

≥2.3

Pediatric reference range has neither been established nor is available in scientific literature. The adult reference range likely would be applicable to children older than 6 months.

Interpretation

An activated protein C (APC) resistance ratio below 2.3 suggests abnormal resistance to APC of hereditary origin.

 

If the screening APC resistance test is abnormal, DNA-based testing for the factor V Leiden variant (p.Arg534Gln, formerly R506Q) is performed to confirm or exclude hereditary APC-resistance.

Clinical Reference

1. Nichols WL, Heit JA. Activated protein C resistance and thrombosis. Mayo Clin Proc. 1996;71(9):897-898

2. Dahlback B. Resistance to activated protein C as risk factor for thrombosis: molecular mechanisms, laboratory investigation, and clinical management. Semin Hematol. 1997;34(3):217-234

3. Rodeghiero F, Tosetto A. Activated protein C resistance and factor V Leiden mutation are independent risk factors for venous thromboembolism. Ann Intern Med. 1999;130(8):643-650. doi:10.7326/0003-4819-130-8-199904200-00004

4. Grody WW, Griffin JH, Taylor AK, Korf BR, Heit JA; ACMG Factor V. Leiden Working Group. American College of Medical Genetics consensus statement on factor V Leiden mutation testing [published correction appears in Genet Med. 2021 Dec;23(12):2463]. Genet Med. 2001;3(2):139-148. doi:10.1097/00125817-200103000-00009 5. Press RD, Bauer KA, Kujovich JL, Heit JA. Clinical utility of factor V Leiden (R506Q) testing for the diagnosis and management of thromboembolic disorders. Arch Pathol Lab Med. 2002;126(11):1304-1318. doi:10.5858/2002-126-1304-CUOFVL

6. Yohe S, Olson J: Thrombophilia: Assays and interpretation. In: Kottke-Marchant K, Davis B, eds. Laboratory Hematology Practice. Blackwell Publishing; 2012:492-508

Day(s) Performed

Monday through Friday

Report Available

4 to 7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

85307

LOINC Code Information

Test ID Test Order Name Order LOINC Value
APCRR APCRV, w/Reflex, P 13590-5

 

Result ID Test Result Name Result LOINC Value
APCR APCRV Ratio 13590-5
SC018 Whole Blood No LOINC Needed
INT55 Interpretation 48591-2

Forms

1. New York Clients-Informed consent is required. Document on the request form or electronic order that a copy is on file. The following documents are available:

-Informed Consent for Genetic Testing (T576)

-Informed Consent for Genetic Testing-Spanish (T826)

2. Coagulation Patient Information (T675)

3. If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.

Test Classification

See Individual Test IDs