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Test Code AMISO Amylase, Isoenzymes, Serum


Necessary Information


Age and sex of patient are required.



Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Serum gel tubes should be centrifuged within 2 hours of collection.

2. Red-top tubes should be centrifuged and the serum aliquoted into a plastic vial within 2 hours of collection.


Useful For

Ruling out salivary amylase as the cause of elevated serum amylase

Profile Information

Test ID Reporting Name Available Separately Always Performed
AMYSE Amylase, Total, S Yes, (Order AMS) Yes
AMYPA Amylase, Pancreatic, S No Yes
AMYSA Amylase, Salivary, S No Yes

Testing Algorithm

Total and pancreatic amylase are measured in the submitted serum specimen. Salivary amylase is calculated as the difference between the two measured results (salivary amylase = total amylase-pancreatic amylase).

Method Name

AMYSE, AMYPA: Colorimetric Rate Reaction

AMYSA: Calculation

Reporting Name

Amylase, Isoenzymes, S

Specimen Type

Serum

Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 30 days
  Frozen  30 days
  Ambient  7 days

Reference Values

AMYLASE, TOTAL

0-30 days: ≤6 U/L

31-182 days: 1-17 U/L

183-365 days: 6-44 U/L

1-3 years: 8-79 U/L

4-17 years: 21-110 U/L

≥18 years: 28-100 U/L

 

AMYLASE, PANCREATIC

0-<24 months: ≤20 U/L

2-<18 years: 9-35 U/L

≥18 years: 13-53 U/L

 

AMYLASE, SALIVARY

0-<18 years: Not established

≥18 years: ≤86 U/L

Interpretation

Increased concentrations of total amylase activity in conjunction with increased concentration of specific amylase isoenzymes may aid in differentiating the source of amylase (pancreatic versus salivary).

Clinical Reference

1. Panteghini M. Laboratory evaluation of pancreatic diseases. Biochimica Clinica. 2010;34(1):19-25

2. Rifai N, Horvath AR, Wittwer CT. Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics. 8th ed. Elsevier; 2018

3. Pieper-Bigelow C, Strocchi A, Levitt MD. Where does serum amylase come from and where does it go? Gastroenterol Clin North Am. 1990;19(4):793-810

4. Azzopardi E, Lloyd C, Teixeira SR, Conlan RS, Whitaker IS. Clinical applications of amylase: Novel perspectives. Surgery. 2016;160(1):26-37

Day(s) Performed

Monday through Sunday

Report Available

1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

82150 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
AMISO Amylase, Isoenzymes, S 24333-7

 

Result ID Test Result Name Result LOINC Value
AMYPA Amylase, Pancreatic, S 1805-1
AMYSA Amylase, Salivary, S 1809-3
AMYSE Amylase, Total, S 1798-8

Forms

If not ordering electronically, complete, print, and send Gastroenterology and Hepatology Test Request (T728) with the specimen.