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Test Code AFH Factor H Autoantibody, Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. Immediately after specimen collection, place the tube on wet ice.

2. After sample has clotted on wet ice, centrifuge at 4° C and aliquot serum into a plastic vial.

3. Freeze specimen within 30 minutes of centrifugation. Sample must be placed on dry ice if not frozen immediately.

Additional Information: If the specimen is to be shared with AHUSD / Atypical Hemolytic Uremic Syndrome Complement Panel, Serum and Plasma, only serum collected in a red-top tube is acceptable.

NOTE: If a refrigerated centrifuge is not available, it is acceptable to use a room temperature centrifuge, provided the sample is kept on ice before centrifugation, and immediately afterward, the serum aliquoted and frozen..


Useful For

Detection and quantification of antibodies to factor H

 

Monitoring patients with known factor H autoantibodies

 

Aiding in the differential diagnosis of thrombotic microangiopathy and C3 glomerulopathies

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

Factor H Autoantibody, S

Specimen Type

Serum Red

Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Red Frozen (preferred) 28 days
  Refrigerated  28 days
  Ambient  14 days

Reference Values

<15.8 U/mL

Interpretation

Absent (<15.8 U/mL): Antibodies to factor H are not detected.

 

Present (≥15.8 U/mL): Antibodies to factor H are detected. Clinical correlation recommended.

Clinical Reference

1. Ekdahl KN, Persson B, Mohlin C, Sandholm K, Skattum L, Nilsson B. Interpretation of serological complement biomarkers in disease. Front Immunol. 2018;9:2237. doi:10.3389/fimmu.2018.02237

2. Jozsi M, Licht C, Strobel S, et al. Factor H autoantibodies in atypical hemolytic uremic syndrome correlate with CFHR1/CFHR3 deficiency. Blood. 2008;111(3):1512-1514. doi:10.1182/blood-2007-09-109876

3. Holmes LV, Strain L, Staniforth SJ, et al. Determining the population frequency of the CFHR3/CFHR1 deletion at 1q32. PLoS One. 2013;8(4):e60352. doi:10.1371/journal.pone.0060352

4. Sinha A, Gulati A, Saini S, et al. Prompt plasma exchanges and immunosuppressive treatment improves the outcomes of anti-factor H autoantibody-associated hemolytic uremic syndrome in children. Kidney Int. 2014;85(5):1151-1160. doi:10.1038/ki.2013.373

5. Durey MA, Sinha A, Togarsimalemath SK, Bagga A. Anti-complement-factor H-associated glomerulopathies. Nat Rev Nephrol. 2016;12(9):563-578. doi:10.1038/nrneph.2016.99

6. Blanc C, Togarsimalemath SK, Chauvet S, et al. Anti-factor H autoantibodies in C3 glomerulopathies and in atypical hemolytic uremic syndrome: one target, two diseases. J Immunol. 2015;194(11):5129-5138. doi:10.4049/jimmunol.1402770

7. Zhang Y, Ghiringhelli Borsa N, Shao D, et al. Factor H autoantibodies and complement-mediated diseases. Front Immunol. 2020;11:607211. doi:10.3389/fimmu.2020.607211

8. Sanchez-Corral P, Pouw RB, Lopez-Trascasa M, Jozsi M. Self-damage caused by dysregulation of the complement alternative pathway: Relevance of the factor H protein family. Front Immunol. 2018;9:1607. doi:10.3389/fimmu.2018.01607

9. Dragon-Durey MA, Blanc C, Roumenina LT, et al. Anti-factor H autoantibodies assay. Methods Mol Biol. 2014;1100:249-56. doi:10.1007/978-1-62703-724-2_20

Day(s) Performed

Monday

Report Available

2 to 8 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

83520

LOINC Code Information

Test ID Test Order Name Order LOINC Value
AFH Factor H Autoantibody, S 101863-9

 

Result ID Test Result Name Result LOINC Value
AFH Factor H Autoantibody, S 101863-9