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HelpTest Code ADNAP Double-Stranded DNA (dsDNA) Antibodies Panel, IgG, Serum
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL Serum
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Useful For
Evaluation of patients at-risk of systemic lupus for erythematosus (SLE) with abnormal antinuclear antibodies
Management of affected individuals
Distinguishing SLE from other autoimmune diseases
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ADNA1 | dsDNA Ab, IgG, S | Yes | Yes |
| CRTHS | dsDNA Ab IFA Screen, IgG, S | Yes | Yes |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| CRTHT | dsDNA Ab IFA Titer, IgG, S | No | No |
Testing Algorithm
If the double-stranded DNA (dsDNA) IgG antibodies Crithidia luciliae immunofluorescence screen is positive, then the dsDNA IgG antibodies Crithidia luciliae immunofluorescence titer will be performed at an additional charge.
For more information see Connective Tissue Disease Cascade.
Method Name
ADNA1: Enzyme-Linked Immunosorbent Assay (ELISA)
CRTHS, CRTHT: Indirect Immunofluorescence Assay (IFA)
Reporting Name
dsDNA Ab Panel, IgG, SSpecimen Type
SerumSpecimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 7 days |
| Frozen | 21 days |
Reference Values
DOUBLE-STRANDED DNA (dsDNA) ANTIBODIES, IgG:
<100 IU/mL (Negative)
≥100 IU/mL (Positive)
Negative is considered normal.
Reference values apply to all ages.
DOUBLE-STRANDED DNA (dsDNA) IgG ANTIBODIES, CRITHIDIA LUCILIAE IMMUNOFLUORESCENCE SCREEN
Negative (<1:10)
Reference values apply to all ages.
DOUBLE-STRANDED DNA (dsDNA) IgG ANTIBODIES, CRITHIDIA LUCILIAE IMMUNOFLUORESCENCE TITER
Negative (<1:10)
Interpretation
The presence of anti-double stranded DNA (anti-dsDNA) IgG antibody is a diagnostic criterion of systemic lupus erythematosus (SLE). Utilization of two different methods to detect anti-dsDNA antibodies has the potential to optimize the diagnosis of SLE and management of affected patients.
Negative results for both the anti-dsDNA IgG by enzyme linked immunosorbent assay and indirect immunofluorescent assay do not exclude a diagnosis of SLE.
Clinical Reference
1. Kavanaugh AF, Solomon DH; American College of Rheumatology Ad Hoc Committee on Immunologic Testing Guidelines. Guidelines for immunologic laboratory testing in the rheumatic diseases: anti-DNA antibody tests. Arthritis Rheum. 2002;47(5):546-555. doi:10.1002/art.10558
2. Petri M, Orbai AM, Alarcon GS, et al. Derivation and validation of the Systemic Lupus International Collaborating Clinics classification criteria for systemic lupus erythematosus. Arthritis Rheum. 2012;64(8):2677-2686. doi:10.1002/art.34473
3. Aringer M, Costenbader K, Daikh D, et al. 2019 European League Against Rheumatism/American College of Rheumatology Classification Criteria for systemic lupus erythematosus. Arthritis Rheumatol. 2019;71(9):1400-1412
4. Compagno M, Rekvig OP, Bengtsson AA, et al. Clinical phenotype associations with various types of anti-dsDNA antibodies in patients with recent onset of rheumatic symptoms. Results from a multicentre observational study. Lupus Sci Med. 2014;1(1):e000007
5. Enocsson H, Sjowall C, Wirestam L, et al. Four anti-dsDNA antibody assays in relation to systemic lupus erythematosus disease specificity and activity. J Rheumatol. 2015;42(5):817-825
6. Sarbu MI, Salman-Monte TC, Rubio Munoz P, Lisbona MP, Almirall Bernabe M, Carbonell J. Differences between clinical and laboratory findings in patients with recent diagnosis of SLE according to the positivity of anti-dsDNA by the Crithidia luciliae method. Lupus. 2015;24(11):1198-1203
7. Zaminski D, Saxena A, Izmirly P, Buyon JP, Belmont HM. Clinical implications of discordance between anti-dsDNA antibodies by multiplex flow immunoassay and Crithidia luciliae assay in a multiethnic racial cohort of patients with SLE. Lupus Sci Med. 2023;10(2):e001012
8. Cockx M, Van Hoovels L, De Langhe E, et al. Laboratory evaluation of anti-dsDNA antibodies. Clin Chim Acta. 2022;528:34-43
Day(s) Performed
ADNA1: Monday through Saturday
CRTHS, CRTHT: Monday through Friday
Report Available
2 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
ADNA1-86225
CRTHS-86255
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| ADNAP | dsDNA Ab Panel, IgG, S | 97564-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| ADNA1 | dsDNA Ab, IgG, S | 33799-8 |
| 623426 | dsDNA Ab IFA Screen, IgG, S | 6457-6 |
| 623463 | dsDNA Interpretation | 69048-7 |