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Test Code C3FX C3 Complement, Functional, Serum

Important Note

  • MUST be drawn at the Outpatient Lab at the Main Hospital
  • MUST be send down on ICE

Reporting Name

C3 Complement, Functional, S

Useful For

Diagnosis of C3 deficiency

 

Investigation of a patient with undetectable total complement level

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Ordering Guidance


The total complement assay (COM / Complement, Total, Serum) should be used as a screen for suspected complement deficiencies before ordering individual complement component assays. A deficiency of an individual component of the complement cascade will result in an undetectable total complement level.



Specimen Required


Patient Preparation:

Fasting: 12 hours, preferred but not required

Supplies: Sarstedt Aliquot Tube 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Immediately after specimen collection, place the tube on wet ice and allow specimen to clot.

2. Centrifuge at 4° C and aliquot serum into a plastic vial.

3. Within 30 minutes of centrifugation, freeze specimen. Specimen must be placed on dry ice if not frozen immediately.

NOTE: If a refrigerated centrifuge is not available, it is acceptable to use a room temperature centrifuge, provided the specimen is kept on ice before centrifugation, and immediately afterward, the serum is aliquoted and frozen.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Frozen 14 days

Reference Values

21-50 U/mL

Day(s) Performed

Tuesday, Friday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

86161

LOINC Code Information

Test ID Test Order Name Order LOINC Value
C3FX C3 Complement, Functional, S 87723-3

 

Result ID Test Result Name Result LOINC Value
C3FX C3 Complement, Functional, S 87723-3

Interpretation

Low levels of complement may be due to inherited deficiencies, acquired deficiencies, or due to complement consumption (eg, as a consequence of infectious or autoimmune processes).

 

Absent C3 levels in the presence of other normal complement values are consistent with a C3 deficiency.

Clinical Reference

1. Davis ML, Austin C, Messmer BL, et al. IFCC-standardization pediatric reference intervals for 10 serum proteins using the Beckman Array 360 system. Clin Biochem. 1996;29(5):489-492

2. Gaither TA, Frank MM. Complement. In: Henry JB, ed. Clinical Diagnosis and Management by Laboratory Methods. 17th ed. WB Saunders Company; 1984:879-892

3. O'Neil KM. Complement deficiency. Clin Rev Allergy Immunol. 2000;19(2):83-108

4. Frank MM. Complement deficiencies. Pediatr Clin North Am. 2000;47(6):1339-1354

5. Brodszki N, Frazer-Abel A, Grumach AS, et al. European Society for Immunodeficiencies (ESID) and European Reference Network on Rare Primary Immunodeficiency, Autoinflammatory and Autoimmune Diseases (ERN RITA) Complement Guideline: Deficiencies, diagnosis, and management. J Clin Immunol. 2020;40(4):576-591

6. Willrich MAV, Braun KMP, Moyer AM, Jeffrey DH, Frazer-Abel A. Complement testing in the clinical laboratory. Crit Rev Clin Lab Sci. 2021;58(7):447-478. doi:10.1080/10408363.2021.1907297

Report Available

1 to 3 days

Method Name

Automated Liposome Lysis Assay